What is the novel coronavirus test for 2019-nCoV?
Real time RT-PCR is the most commonly used method to detect the nucleic acid of pathogens, and sara-cov-2 is no exception. At present, there are also some isothermal amplification methods and some direct hybridization methods. But no matter what method, as long as the detection of viral nucleic acid, usually need primers and probes. The similarity of the novel coronavirus to the SARS coronavirus is higher than that of the other two, which is close to 80%. This is similar to other coronavirus, and its consistency with bat coronavirus is the highest, reaching over 95%. The similarity of the different regions of about 30000 bases is different from that of other coronaviruses, and the similarity of some regions is more than 90%. However, in order to ensure the specificity of detection, the nucleic acid detection reagent must select the regions with low similarity to design primers and probes, so once detected, it is "positive", proving that there is a virus, that is, the existence of infection. Therefore, novel coronavirus positive detection can be used as the gold standard for the diagnosis of new coronavirus infection.
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