See that the nucleic acid amplification kits of the company all contain Rox correction system:
Correct signal changes that may be caused by bubbles or evaporation / concentration during operation;
Provide powerful fault diagnosis tools: passively correct the signal, not affected by the reaction, collect the signal in each cycle of operation;
Correct volume differences that may be caused by sample addition or possible evaporation in one operation;
Correct volume differences between multiple runs to provide more consistent CT values between runs.